1x pcr reaction buffer Search Results


pcr reaction buffer [1x]  (GenScript corporation)
90
GenScript corporation pcr reaction buffer [1x]
Pcr Reaction Buffer [1x], supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr reaction buffer [1x]/product/GenScript corporation
Average 90 stars, based on 1 article reviews
pcr reaction buffer [1x] - by Bioz Stars, 2026-03
90/100 stars
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1x pcr reaction buffer altona diagnostics  (Altona Diagnostics)
90
Altona Diagnostics 1x pcr reaction buffer altona diagnostics
Relation between aptamer concentration and cycle threshold ( C t ) at 31 and 55 °C. Aptamer concentrations of 0, 12.5, 25, and 50 μM per reaction ( a ) and 0, 50, 100, and 200 μM per reaction ( b ) were tested in a one-step <t>real-time</t> <t>RT-PCR</t> assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using 1000 <t>RNA</t> copies per reaction. RT-step was carried out for 20 min in parallel at 31 °C ( circles ) and 55 °C ( dots ), respectively. Results of 200 μM aptamer concentration at 31 °C did not lead to a positive signal and therefore are not shown
1x Pcr Reaction Buffer Altona Diagnostics, supplied by Altona Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x pcr reaction buffer altona diagnostics/product/Altona Diagnostics
Average 90 stars, based on 1 article reviews
1x pcr reaction buffer altona diagnostics - by Bioz Stars, 2026-03
90/100 stars
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1x pcr reaction buffer  (Beijing Solarbio Science)
90
Beijing Solarbio Science 1x pcr reaction buffer
Relation between aptamer concentration and cycle threshold ( C t ) at 31 and 55 °C. Aptamer concentrations of 0, 12.5, 25, and 50 μM per reaction ( a ) and 0, 50, 100, and 200 μM per reaction ( b ) were tested in a one-step <t>real-time</t> <t>RT-PCR</t> assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using 1000 <t>RNA</t> copies per reaction. RT-step was carried out for 20 min in parallel at 31 °C ( circles ) and 55 °C ( dots ), respectively. Results of 200 μM aptamer concentration at 31 °C did not lead to a positive signal and therefore are not shown
1x Pcr Reaction Buffer, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x pcr reaction buffer/product/Beijing Solarbio Science
Average 90 stars, based on 1 article reviews
1x pcr reaction buffer - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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Relation between aptamer concentration and cycle threshold ( C t ) at 31 and 55 °C. Aptamer concentrations of 0, 12.5, 25, and 50 μM per reaction ( a ) and 0, 50, 100, and 200 μM per reaction ( b ) were tested in a one-step real-time RT-PCR assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using 1000 RNA copies per reaction. RT-step was carried out for 20 min in parallel at 31 °C ( circles ) and 55 °C ( dots ), respectively. Results of 200 μM aptamer concentration at 31 °C did not lead to a positive signal and therefore are not shown

Journal: Journal of Analytical Science and Technology

Article Title: Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance

doi: 10.1186/s40543-015-0063-4

Figure Lengend Snippet: Relation between aptamer concentration and cycle threshold ( C t ) at 31 and 55 °C. Aptamer concentrations of 0, 12.5, 25, and 50 μM per reaction ( a ) and 0, 50, 100, and 200 μM per reaction ( b ) were tested in a one-step real-time RT-PCR assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using 1000 RNA copies per reaction. RT-step was carried out for 20 min in parallel at 31 °C ( circles ) and 55 °C ( dots ), respectively. Results of 200 μM aptamer concentration at 31 °C did not lead to a positive signal and therefore are not shown

Article Snippet: The one-step real-time RT-PCR was performed in a 25-μL reaction mix containing 10 μL of RNA template, 1x PCR reaction buffer (altona Diagnostics GmbH), 2.4 mM MgCl 2 (Sigma-Aldrich), 240 μg/μL BSA (Roche), 1 U of Platinum® Taq DNA Polymerase high fidelity (Invitrogen), 156 U of SuperScript® III Reverse Transcriptase (Invitrogen).

Techniques: Concentration Assay, Quantitative RT-PCR

Probit regression analyses of a real-time RT-PCR with 25 μM aptamer and without aptamer. Probit regression analysis was carried out with target RNA loads from 10 −1 to 10 2 copies per reaction. Each dilution was analyzed in four independent runs with six replicates. The target RNA concentration is plotted on the X-axis, and the Y-axis displays the hit rate. Circles are experimental data points; the inner lines represent the corresponding probit curve, outer lines the 95 % confidence intervals. a Without aptamer; b 25 μM aptamer

Journal: Journal of Analytical Science and Technology

Article Title: Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance

doi: 10.1186/s40543-015-0063-4

Figure Lengend Snippet: Probit regression analyses of a real-time RT-PCR with 25 μM aptamer and without aptamer. Probit regression analysis was carried out with target RNA loads from 10 −1 to 10 2 copies per reaction. Each dilution was analyzed in four independent runs with six replicates. The target RNA concentration is plotted on the X-axis, and the Y-axis displays the hit rate. Circles are experimental data points; the inner lines represent the corresponding probit curve, outer lines the 95 % confidence intervals. a Without aptamer; b 25 μM aptamer

Article Snippet: The one-step real-time RT-PCR was performed in a 25-μL reaction mix containing 10 μL of RNA template, 1x PCR reaction buffer (altona Diagnostics GmbH), 2.4 mM MgCl 2 (Sigma-Aldrich), 240 μg/μL BSA (Roche), 1 U of Platinum® Taq DNA Polymerase high fidelity (Invitrogen), 156 U of SuperScript® III Reverse Transcriptase (Invitrogen).

Techniques: Quantitative RT-PCR, Concentration Assay